Journal: Journal of medical virology

Article Title: SARS-CoV-2 pseudovirus infectivity and expression of viral entry-related factors ACE2, TMPRSS2, Kim-1 and NRP-1 in human cells from respiratory, urinary, digestive, reproductive and immune systems

doi: 10.1002/jmv.27244

Figure Lengend Snippet: Cell lines used in the present study

Article Snippet: Table 1 Cell name Origin Source of cell Culture medium Respiratory system JHU‐029 Laryngeal squamous cell carcinoma CVCL_5993 RPMI‐1640 complete medium NCL H460 Large cell lung cancer ATCC HTB‐177 RPMI‐1640 complete medium NCL H322 Large cell lung cancer ATCC CRL­5806 DMEM complete medium NCL H520 Large cell lung cancer ATCC HTB‐182 DMEM complete medium A549 Lung adenocarcinoma ATCC CRM‐CCL‐185 DMEM complete medium Primary human lobar bronchial epithelial cells (HLBEC) Lobar bronchial tissue Lifeline® Cell Technology Lifeline® BronchiaLife™ Medium Primary human small airway epithelial cells (HSAEC) Small airway tissue Lifeline® Cell Technology Lifeline® BronchiaLife™ Medium Urinary system 769‐P Renal cell adenocarcinoma ATCC CRL‐1933 RPMI‐1640 complete medium 768‐O Renal cell adenocarcinoma ATCC CRL‐1932 RPMI‐1640 complete medium A498 Renal cell adenocarcinoma ATCC HTB‐44 RPMI‐1640 complete medium Caki‐1 Renal cell carcinoma ATCC HTB‐46 DMEM complete medium ACHN Renal cell adenocarcinoma ATCC CRL‐1611 DMEM complete medium HRC45 Renal cell carcinoma CVCL_IS24 DMEM complete medium HRC63 Renal cell carcinoma CVCL_IS25 DMEM complete medium HRC59 Renal cell carcinoma FCCC DMEM complete medium Immune system BCP‐1 Primary effusion lymphoma ATCC CRL‐2294 RPMI‐1640 complete medium BC‐3 Primary effusion lymphoma ATCC CRL‐2277 RPMI‐1640 complete medium BJAB Primary effusion lymphoma CVCL_5711 RPMI‐1640 complete medium THP‐1 Acute monocytic leukemia ATCC TIB‐202 RPMI‐1640 complete medium Digestive system Huh‐7 Hepatocellular carcinoma cell JCRB0403 DMEM complete medium PCI‐13 Oral cavity squamous cell carcinoma CVCL_C182 RPMI‐1640 complete medium UD‐SCC‐2 Hypopharyngeal squamous cell carcinoma CVCL_E325 RPMI‐1640 complete medium Reproductive system HUVEC Umbilical Vein Endothelial Cells ATCC PCS‐100‐010 ECBM(Cell applications, 210‐490) T47D Ductal carcinoma ATCC HTB‐133 RPMI‐1640 complete medium MCF‐7 Breast adenocarcinoma ATCC HTB‐22 RPMI‐1640 complete medium Open in a separate window Note : DMEM or RPMI‐1640 complete medium is made of DMEM or RPMI‐1640 basal medium with 10% fetal bovine serum (FBS) and 1% Penicillin‐Streptomycin 100× Solution (25‐512, GenClone).

Techniques: Cell Culture

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: Construction of HUVEC oxidative stress and inflammatory injury models. A: HUVECs were cultured with 100 mg/L oxidized low-density lipoprotein (ox-LDL) for 24 h to establish an oxidative stress injury model. Cell proliferation was detected by CCK8 assays, and cell apoptosis was determined using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). B: HUVECs were cultured with 50 ng/mL tumor necrosis factor- α (TNF- α ) for 24 h to establish an inflammatory injury model, Cell proliferation was measured by CCK8 assay, and cell apoptosis assay was determined with Annexin V-FITC/PI staining followed by flow cytometry analysis ( n = 3). ∗ compared with Normal group; ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Cell Culture, Staining, Flow Cytometry, CCK-8 Assay, Apoptosis Assay

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: M2 macrophages enhanced HUVEC cell proliferation and inhibited HUVEC cell apoptosis in the HUVEC oxidative stress injury model. (a, c) Cell apoptosis assays were performed using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). (b) Cell proliferation was determined using CCK8 assays. HUVECs were treated with or without M0, M1, or M2 macrophages ( n = 3). (d) qRT-PCR analysis of mRNA levels of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α mRNA in HUVECs ( n = 3). (e) Representative western blots of apoptosis-related protein (Caspase-3, Bcl2, and c-myc) expressions in HUVECs after macrophage stimulation ( n = 3). ∗ Compared with control group. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Staining, Flow Cytometry, Quantitative RT-PCR, Western Blot, Control

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: M2 macrophages enhanced HUVEC proliferation and inhibited HUVEC cell apoptosis in the HUVEC inflammatory injury model. (a, c) Cell apoptosis assay was determined using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). (b) Cell proliferation was determined using CCK8 assays. HUVECs were treated with or without M0, M1, or M2 macrophages ( n = 3). (d) qRT-PCR analyses of mRNA levels of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α in HUVECs ( n = 3). (e) Representative western blots of apoptosis-related protein (Caspase-3, Bcl2, and c-myc) expressions in HUVEC cells after macrophage stimulation ( n = 3). ∗ Compared with control group. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Apoptosis Assay, Staining, Flow Cytometry, Quantitative RT-PCR, Western Blot, Control

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: M2 macrophage Exos enhanced HUVEC cell proliferation and inhibited HUVEC cell apoptosis in the HUVEC cell oxidative stress injury model. (a, c) Cell apoptosis assay was determined using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). (b) Cell proliferation was determined using CCK8 assays. HUVECs were treated with or without M0, M1, or M2 macrophage Exos ( n = 3). (d) qRT-PCR analyses of mRNA levels of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α mRNA in HUVECs ( n = 3). (e) Representative western blots of apoptosis-related protein (Caspase-3, Bcl2, and c-myc) expressions in HUVEC cells after Exos stimulation ( n = 3). ∗ Compared with control group. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Apoptosis Assay, Staining, Flow Cytometry, Quantitative RT-PCR, Western Blot, Control

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: M2 macrophage Exos enhanced HUVEC cell proliferation and inhibited HUVEC cell apoptosis in the HUVEC cell inflammatory injury model. (a, c) Cell apoptosis assay was determined using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). (b) Cell proliferation was determined using CCK8 assay. HUVECs were treated with or without M0, M1, or M2 macrophage Exos ( n = 3). (d) qRT-PCR analyses of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α mRNA in HUVECs ( n = 3). (e) Representative western blots of apoptosis-related protein (Caspase-3, Bcl2, and c-myc) expressions in HUVEC cells after Exos stimulation ( n = 3). ∗ Compared with control group. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Apoptosis Assay, Staining, Flow Cytometry, CCK-8 Assay, Quantitative RT-PCR, Western Blot, Control

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: Effect of miR-221-3p in the HUVEC oxidative stress injury model. (a) The relative miRNA expression of miR-4449, miR-211-5p, miR-10b-3p, miR-503-5p, miR-708-5p, and miR-221-3p in macrophages and macrophage Exos ( n = 3). (b) The relative miRNA expression of miR-221-3p after transfection ( n = 3). (c, e) Cell apoptosis assay was determined using Annexin V-FITC/PI staining followed by flow cytometry ( n = 3). (d) Cell proliferation was determined by CCK8 assay ( n = 3). (f) qRT-PCR analyses of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α mRNA in HUVECs ( n = 3). (g) Representative western blots of apoptosis-related proteins (Caspase-3, Bcl2, and c-myc) and Grb10 protein expression in HUVEC cells after liposome transfection ( n = 3). ∗ Compared with control group; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Expressing, Transfection, Apoptosis Assay, Staining, Flow Cytometry, CCK-8 Assay, Quantitative RT-PCR, Western Blot, Control

Journal: BioMed Research International

Article Title: M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

doi: 10.1155/2022/1609244

Figure Lengend Snippet: Effect of miR-221-3p in the HUVEC inflammatory injury model. (a, c) Cell apoptosis assay was determined using Annexin V/PI staining followed by flow cytometry ( n = 3). (b) Cell proliferation was determined by CCK8 assay ( n = 3). (d) qRT-PCR analyses of inflammatory factors IL-6, IL-10, IL-1 β , and TNF- α mRNA in HUVECs ( n = 3). (e) Representative western blots of apoptosis-related proteins (Caspase-3, Bcl2, and c-myc) and Grb10 protein expression in HUVEC cells after liposome transfection ( n = 3). ∗ Compared with control group. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: THP-1 cells were cultured in BPMI 1640 medium (TBD, Tianjin, China) with 10% fetal bovine serum (Procell, Wuhan, China), and HUVECs were cultured in a primary HUVEC cell culture system (iCell, Shanghai, China) with 10% fetal bovine serum.

Techniques: Apoptosis Assay, Staining, Flow Cytometry, CCK-8 Assay, Quantitative RT-PCR, Western Blot, Expressing, Transfection, Control